Data and Methodology

• Current Animal Models
  View Histology, Physiology, Analyzed Microarray Data, and Related Literature.
• Downloadable Microarray Data
  Download Raw Affymetrix Microarray Files or Tab Delimited Text Files.
• Quantitative PCR DB
  Find and add working probes for Quantitative PCR.
• Performance Progress
  Summary of Each Animal Model's Progress to Date.
• Methodology
  Learn about our protocol and RNA Expression Profiling using Affymetrix
• Quality Control (QC)/ Quality Assurance (QA)
• ChipperDB - MIAME (Minimum Information About a Microarray Experiment) database
  Physiological, RNA Isolation and Labeling, and Hybridization Information
• Bioinformatics Links - freely available bioinformatics tools for analyzing Affymetrix data

Education

• Interactive web tutorial on Myocardial Infarction
  * Note: this animation requires the Flash 6 player.
  Test for the Flash Player | Download Flash Player

Coming:

• Analyzed Microarray Data for IGF dataset
• Analyzed Microarray Data for Embryo dataset

Overview of Project 1:

Our goal is to build a global picture of the transcriptional regulatory network during cardiac hypertrophy and failure. Candidate genes that are identified in this study will then undergo SNP analysis by the Whitehead Genome Center in the Framingham Heart Study population (see Project 5).

This PGA is designed to generate a well characterized "benchmark" data set for each mouse model, consisting of gene expression profiles, histological characterization, and physiological data. The data generated by all of the above studies will be analyzed using state-of-the-art tools of informatics, extensively annotated and made freely available to the research community through an interactive website.

We hope that these resources together with your research will lead to new hypotheses, a better understanding of the disease process, and ultimately the identification of novel therapeutic targets in the prevention and treatment of human heart failure.

Top of page