Histology

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Morphological analysis of homozygous Nkx2.5 null embryos. (A) Wild-type (+/+) and homozygous mutant (-/-) embryos at 10.5 d.p.c. The arrowhead shows the pericardium of the mutant embryo. (B) Whole-mount b-gal staining of a homozygous mutant embryo at 9.5 d.p.c. (C) Schematic representation of wild-type (+/+) and homozygous mutant (-/-) hearts at 9.5 d.p.c. RV, right ventricle; LV, left ventricle; V, single ventricle. (D-G) Scanning electron micrograms of wild-type (D,E) and homozygous mutant (F,G) embryos at 9.5 d.p.c. Left lateral views (D,F) and ventral views (E,G). The pericardium was removed to show heart structures. The atrioventricular canal was wide open and the bulboventicular sulcus did not form in the homozygous mutant embryo. a, atrium; v, ventricle; rv, right ventricle; lv, left ventricle; ot, outflow tract. (H,I) H&E-stained transverse sections of wild-type (H) and homozygous mutant (I) embryos at 9.5 d.p.c. Note the trabeculation (arrows) and endocardial cushion formation (arrowheads) in the wild-type embryo. (J) Whole-mount PECAM staining of wild-type and homozygous mutant embryos at 9.5 d.p.c. Note poor development of intersomitic arteries, dorsal aorta and pharyngeal arch arteries in the mutant embryo. Taken from (Tanaka et al., 1999)

Page last modified: 11-Mar-2004